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Rapid metabolic profiling of developing Pseudomonas aeruginosa biofilms by high-resolution mass spectrometry fingerprinting

Rapid metabolic profiling of developing Pseudomonas aeruginosa biofilms by high-resolution mass spectrometry fingerprinting

Kategori
Vitenskapelig artikkel
Sammendrag
Biofilms are single- or multi-species communities of bacteria that are enclosed in an extracellular matrix. These cells generally exhibit phenotypes that are significantly different from those of planktonic cells, yet detailed elucidation of the causality and the exact nature of this metabolic shift remains challenging. Considering the strong correlation of biofilms with pathogenicity and disease in the clinic, as well as the veritable economic impact of biofilms in other areas, a methodology for in-vivo monitoring of biofilm development is necessary. Here, we present high-resolution mass spectrometry fingerprinting as a rapid, sensitive, and generic technique for online, non-invasive monitoring of developing biofilms. The opportunistic pathogen Pseudomonas aeruginosa is used as a model system, and it is demonstrated that strain- and time-dependent changes in biofilm extracellular metabolites are easily detected.
Språk
Engelsk
Forfatter(e)
Institusjon(er)
  • SINTEF Industri / Bioteknologi og nanomedisin
  • Universitetet i Bergen
  • SINTEF Ocean / Miljø og nye ressurser
  • Norges teknisk-naturvitenskapelige universitet
År
2014
Publisert i
Annals of Microbiology
ISSN
1590-4261
Forlag
BioMed Central (BMC)
Årgang
65
Hefte nr.
2
Side(r)
891 - 898