Publikasjoner og ansvarsområder
Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains
L-lysine production by Bacillus methanolicus: Genome-based mutational analysis and L-lysine secretion engineering
Bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. This bacterium was previously applied for methanol-based production of l-glutamate, l-lysine and the five-carbon diamine cadaverine by wild type, classical muta...
6-Phosphofructokinase and ribulose-5-phosphate 3-epimerase in methylotrophic Bacillus methanolicus ribulose monophosphate cycle
...d-Ribulose-5-phosphate-3-epimerase (RPE) and 6-phosphofructokinase (PFK) catalyse two reactions in the ribulose monophosphate (RuMP) cycle in Bacillus methanolicus. The B. methanolicus wild-type strain MGA3 possesses two putative rpe and pfk genes encoded on plasmid pBM19 (rpe1-MGA3 and pfk1-MGA3) a...
Genome-based genetic tool development for Bacillus methanolicus: Theta-and rolling circle-replicating plasmids for inducible gene expression and application to methanol-based cadaverine production
Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter and a putative mannitol activator gene mtlR were identified. The mannitol induc...
Methanol-based production of L-lysine and 1,5-diaminopentane
Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains
Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production ...
Thermophilic Bacillus methanolicus, a promising cell factory in the methanol-based bioeconomy
Combinatorial Mutagenesis and Selection of Improved Signal Sequences and Their Application for High-Level Production of Translocated Heterologous Proteins in Escherichia coli
Continuous hydrogen production by sorption enhanced steam methane reforming (SE-SMR) has been studied using a circulating fluidized bed reactor with calcined natural dolomite as CO2 sorbent and Ni/NiAl2O4 as catalyst. A steam to methane ratio of 4 was used at 575 °C and ambient pressure. Two ex...