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Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains

Abstract

Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. This was achieved by the heterologous expression of the Escherichia coli genes cadA and ldcC encoding two different lysine decarboxylase enzymes, and by increasing the overall L-lysine production levels in this host. Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine production levels up to 500 mg l−1 during shake flask conditions. A volume-corrected concentration of 11.3 g l−1 of cadaverine was obtained by high-cell density fed-batch methanol fermentation. Our results demonstrated that efficient conversion of L-lysine into cadaverine presumably has severe effects on feedback regulation of the L-lysine biosynthetic pathway in B. methanolicus. By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable to the well-known bacterial hosts E. coli and Corynebacterium glutamicum. This study represents the first demonstration of microbial production of cadaverine from methanol.

Category

Academic article

Client

  • Research Council of Norway (RCN) / 224973

Language

English

Author(s)

  • Ingemar Nærdal
  • Johannes Pfeifenschneider
  • Trygve Brautaset
  • Volker F. Wendisch

Affiliation

  • SINTEF Industry / Biotechnology and Nanomedicine
  • University of Bielefeld
  • Norwegian University of Science and Technology

Year

2015

Published in

Microbial Biotechnology

ISSN

1751-7907

Publisher

John Wiley & Sons

Volume

8

Issue

2

Page(s)

342 - 350

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