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Cellular responses of human astrocytoma cells to dust from the Acheson process. An in vitro study

Cellular responses of human astrocytoma cells to dust from the Acheson process. An in vitro study

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Vitenskapelig artikkel
Sammendrag
Silicon carbide (SiC) is largely used in various products such as diesel particulate
filters and solar panels. It
is produced through the Acheson process where aerosolized fractions of SiC and other by-products are
generated in the work environment and may potentially affect the workers’ health. In this study, dust was
collected directly on a
filter in a furnace hall over a time period of 24 h. The collected dust was
characterized by scanning electron microscopy and found to contain a high content of graphite particles,
and carbon and silicon containing particles. Only 6% was classified as SiC, whereof only 10% had a
fibrous
structure. To study effects of exposure beyond the respiratory system, neurotoxic effects on human
astrocytic cells, were investigated. Both low, occupationally relevant, and high doses from 9E-6 mg/cm2
up to 4.5 mg/cm2were used, respectively. Cytotoxicity assay indicated no effects of low doses but an effect
of the higher doses after 24 h. Furthermore, investigation of intracellular reactive oxygen species (ROS)
indicated no effects with low doses, whereas a higher dose of 0.9 mg/cm2 induced a significant increase in
ROS and DNA damage. In summary, low doses of dust from the Acheson process may exert no or little
toxic effects, at least experimentally in the laboratory on human astrocytes. However, higher doses have
implications and are likely a result of the complex composition of the dust.
© 2017 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND
Oppdragsgiver
  • Research Council of Norway (RCN) / 245216
Språk
Engelsk
Forfatter(e)
  • Yke Jildouw Arnoldussen
  • Torunn Kringlen Ervik
  • Balazs Berlinger
  • Ida Teresia Kero
  • Sergey Shaposhnikov
  • Shanbeh Zienolddiny
Institusjon(er)
  • Statens arbeidsmiljøinstitutt
  • SINTEF Industri / Metallproduksjon og prosessering
  • Comet BioTech AS
  • Diverse norske bedrifter og organisasjoner
År
2018
Publisert i
Neurotoxicology
ISSN
0161-813X
Forlag
Elsevier
Årgang
65
Side(r)
241 - 247