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Self-assembly and characterization of transferrin-gold nanoconstructs and their interaction with bio-interfaces

Self-assembly and characterization of transferrin-gold nanoconstructs and their interaction with bio-interfaces

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Tidsskriftspublikasjon
Sammendrag
Transferrin (Tf) conjugated to gold nanoparticles and clusters combine the protein's site-specific receptor targeting capabilities with the optical properties imparted by the nano-sized gold. We have described two different synthesis protocols, one yielding fluorescent Tf-stabilized gold nanoclusters (AuNCs) and one yielding Tf-stabilized gold nanoparticles that exhibit localized surface plasmon resonance. We demonstrate that the synthetic route employed has a large influence both on the gold nanostructure formed, and also on the structural integrity of the protein. A slight protein unfolding allows stronger interaction with lipids, and was found to significantly perturb lipid monolayers. Interactions between the protein–gold nanostructures and three different cell types were also assessed, indicating that the enhanced membrane affinity may be attributed to intercellular membrane differences.
Oppdragsgiver
  • Samarbeidsorganet mellom Helse Midt-Norge og NTNU / 46056629
  • Samarbeidsorganet mellom Helse Midt-Norge og NTNU / 46052400
Språk
Engelsk
Forfatter(e)
  • McDonagh Birgitte Hjelmeland
  • Volden Sondre
  • Lystvet Sina Maria
  • Singh Gurvinder
  • Ese Marit-Helen
  • Ryan Joseph A.
  • Lindgren Mikael
  • Sandvig Axel
  • Sandvig Ioanna
  • Glomm Wilhelm
Institusjon(er)
  • Norges teknisk-naturvitenskapelige universitet
  • Norges teknisk-naturvitenskapelige universitet
  • SINTEF Energi AS / Elkraftteknologi
  • Iona College
  • Norges teknisk-naturvitenskapelige universitet
  • Norges teknisk-naturvitenskapelige universitet
  • Umeå universitet
  • University of Cambridge
  • SINTEF Industri / Bioteknologi og nanomedisin
År
2015
Publisert i
Nanoscale
ISSN
2040-3364
Årgang
7
Hefte nr.
17
Side(r)
8062 - 8070