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Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains

Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains

Kategori
Vitenskapelig artikkel
Sammendrag
Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. This was achieved by the heterologous expression of the Escherichia coli genes cadA and ldcC encoding two different lysine decarboxylase enzymes, and by increasing the overall L-lysine production levels in this host. Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine production levels up to 500 mg l−1 during shake flask conditions. A volume-corrected concentration of 11.3 g l−1 of cadaverine was obtained by high-cell density fed-batch methanol fermentation. Our results demonstrated that efficient conversion of L-lysine into cadaverine presumably has severe effects on feedback regulation of the L-lysine biosynthetic pathway in B. methanolicus. By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable to the well-known bacterial hosts E. coli and Corynebacterium glutamicum. This study represents the first demonstration of microbial production of cadaverine from methanol.
Oppdragsgiver
  • Research Council of Norway (RCN) / 224973
Språk
Engelsk
Forfatter(e)
  • Ingemar Nærdal
  • Johannes Pfeifenschneider
  • Trygve Brautaset
  • Volker F. Wendisch
Institusjon(er)
  • SINTEF Industri / Bioteknologi og nanomedisin
  • Universität Bielefeld
  • Norges teknisk-naturvitenskapelige universitet
År
2015
Publisert i
Microbial Biotechnology
ISSN
1751-7907
Forlag
John Wiley & Sons
Årgang
8
Hefte nr.
2
Side(r)
342 - 350