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Storage and reactivation of enzymes in a disposable, self-contained lab-on-a-chip system

Storage and reactivation of enzymes in a disposable, self-contained lab-on-a-chip system

Kategori
Vitenskapelig Kapittel/Artikkel/Konferanseartikkel
Sammendrag
For a point-of-care chip, it is important to have reactants stably stored on-chip. In this study, deposition and drying of a solution of three enzymes have been addressed. The time for diffusion/rehydration of fluorescently labelled mouse IgG in the dried enzyme solution was ~10 minutes. Microchips with native cyclic olefin copolymer (COC) surfaces showed large adsorption of mouse IgG, while polyethylene glycol (PEG) coated surfaces showed adequate protein resistance. Successful amplification on chip in 500 nl reaction chambers was obtained for spotted and dried enzymes when 0.5% PEG 8000 was applied.
Språk
Engelsk
Forfatter(e)
Institusjon(er)
  • SINTEF Digital / Microsystems and Nanotechnology
År
Forlag
Transducers Research Foundations
Bok
Eleventh International Conference on Miniaterized Systems for Chemistry and Life Sciences : Proceedings of MicroTAS 2007, Paris 7-11 October 2007
ISBN
9780979806407
Side(r)
673 - 675