Til hovedinnhold

Mannuronan C-5 Epimerases Suited for Tailoring of Specific Alginate Structures Obtained by High-Throughput Screening of an Epimerase Mutant Library

Mannuronan C-5 Epimerases Suited for Tailoring of Specific Alginate Structures Obtained by High-Throughput Screening of an Epimerase Mutant Library

Kategori
Vitenskapelig artikkel
Sammendrag
The polysaccharide alginate is produced by brown algae and some bacteria and is composed of the two monomers, β-d-mannuronic acid (M) and α-l-guluronic acid (G). The distribution and composition of M/G are important for the chemical-physical properties of alginate and result from the activity of a family of mannuronan C-5 epimerases that converts M to G in the initially synthesized polyM. Traditionally, G-rich alginates are commercially most interesting due to gelling and viscosifying properties. From a library of mutant epimerases we have isolated enzymes that introduce a high level of G-blocks in polyM more efficiently than the wild-type enzymes from Azotobacter vinelandii when employed for in vitro epimerization reactions. This was achieved by developing a high-throughput screening method to discriminate between different alginate structures. Furthermore, genetic and biochemical analyses of the mutant enzymes have revealed structural features that are important for the differences in epimerization pattern found for the various epimerases.
Oppdragsgiver
  • Research Council of Norway (RCN) / 221576
Språk
Engelsk
Forfatter(e)
Institusjon(er)
  • SINTEF Industri / Bioteknologi og nanomedisin
  • Norges teknisk-naturvitenskapelige universitet
  • SINTEF Industri
År
Publisert i
Biomacromolecules
ISSN
1525-7797
Årgang
14
Hefte nr.
8
Side(r)
2657 - 2666