Sammendrag
This work has been focused on the isolation and characterization of microorganism from decayed wood. 357 bacterial species were isolated from different wood samples
and European locations. 25% of them belong to the Bacillus genus, which are known as producer metabolites and enzymes suitable for bioremediation, biodegradation or
biocontrol applications. Competitive bioassays were used to test their capacity to inhibit the growth of fungi involved in wood-decay. Thus, different strains of Bacillus spp. were selected due to their high inhibitory potential.
Growth condition (temperature, agitation, carbon and nitrogen source concentrations) of these strains were optimized by using response surface methodology.
Temperature and concentration of the nitrogen source were the conditions that more directly influence the biomass production.
It was also evaluated the secretion of compound with antibiotic activity. The antifungal effect against the wood-decay fungus Aspergillus brasilienesis was higher when Bacillus sp grew on an specific medium reaching up to 30% of inhibition.
Taking all of this together, the strain, named V17C1, was selected as the most powerful from the Bacillus sp regarding its strong inhibition Therefore, the next step was to sequence its genome, as well as analyze its secretome profile in order to determine which specific genes and compounds are responsible of its antifungal activity.
Special thanks to the EU project ProWood of the ERA-IB 7th call through the APCIN call of the MINECO (Spain) (ID: PCIN-2016-081).
and European locations. 25% of them belong to the Bacillus genus, which are known as producer metabolites and enzymes suitable for bioremediation, biodegradation or
biocontrol applications. Competitive bioassays were used to test their capacity to inhibit the growth of fungi involved in wood-decay. Thus, different strains of Bacillus spp. were selected due to their high inhibitory potential.
Growth condition (temperature, agitation, carbon and nitrogen source concentrations) of these strains were optimized by using response surface methodology.
Temperature and concentration of the nitrogen source were the conditions that more directly influence the biomass production.
It was also evaluated the secretion of compound with antibiotic activity. The antifungal effect against the wood-decay fungus Aspergillus brasilienesis was higher when Bacillus sp grew on an specific medium reaching up to 30% of inhibition.
Taking all of this together, the strain, named V17C1, was selected as the most powerful from the Bacillus sp regarding its strong inhibition Therefore, the next step was to sequence its genome, as well as analyze its secretome profile in order to determine which specific genes and compounds are responsible of its antifungal activity.
Special thanks to the EU project ProWood of the ERA-IB 7th call through the APCIN call of the MINECO (Spain) (ID: PCIN-2016-081).