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Effect of Ultrasound on the Vasculature and Extravasation of Nanoscale Particles Imaged in Real Time

Effect of Ultrasound on the Vasculature and Extravasation of Nanoscale Particles Imaged in Real Time

Category
Journal publication
Abstract
Ultrasound and microbubbles have been found to improve the delivery of drugs and nanoparticles to tumor tissue. To obtain new knowledge on the influence of vascular parameters on extravasation and to elucidate the effect of acoustic pressure on extravasation and penetration of nanoscale particles into the extracellular matrix, real-time intravital multiphoton microscopy was performed during sonication of tumors growing in dorsal window chambers. The impact of vessel diameter, vessel structure and blood flow was characterized. Fluorescein isothiocyanate–dextran (2 MDa) was injected to visualize blood vessels. Mechanical indexes (MI) of 0.2–0.8 and in-house-made, nanoparticle-stabilized microbubbles or Sonovue were applied. The rate and extent of penetration into the extracellular matrix increased with increasing MI. However, to achieve extravasation, smaller vessels required MIs (0.8) higher than those of blood vessels with larger diameters. Ultrasound changed the blood flow rate and direction. Interestingly, the majority of extravasations occurred at vessel branching points.
Client
  • Regionalt helseforetak / 900000
  • Norges forskningsråd / 240316
  • Samarbeidsorganet mellom Helse Midt-Norge og NTNU / 90270600
Language
English
Author(s)
  • Yemane Petros Tesfamichael
  • Åslund Andreas
  • Snipstad Sofie
  • Bjørkøy Astrid
  • Grendstad Kristin
  • Berg Sigrid
  • Mørch Ýrr Asbjørg
  • Torp Sverre Helge
  • Hansen Rune
  • Davies Catharina de Lange
Affiliation
  • Norwegian University of Science and Technology
  • SINTEF Industry / Biotechnology and Nanomedicine
  • St. Olavs Hospital, Trondheim University Hospital
  • St. Olavs Hospital, Trondheim University Hospital
  • Norwegian University of Science and Technology
  • SINTEF Digital / Health Research
  • Norwegian University of Science and Technology
  • St. Olavs Hospital, Trondheim University Hospital
Year
2019
Published in
Ultrasound in Medicine and Biology
ISSN
0301-5629
Volume
45
Booklet
11
Page(s)
3028 - 3041