Abstract
Plastic particulates in the environment pose an increasing concern for regulatory bodies due to their potential risk
to higher organisms (including humans) as they enter the food chain. Nanoplastics (defined here as smaller than
1 μm) are particularly challenging to detect and analyze at environmentally relevant concentrations and in
biological matrices. The tunicate Ciona Robusta is an effective bioindicator for microplastics and nanoplastic
contamination in the marine environment, due to its capacity to filter substantial volumes of water and to
accumulate particulates. In this proof-of-principle study that demonstrates a complete methodology, following
controlled exposure using spiked samples of a model nanoplastic (100 nm diameter polystyrene spheres) the
nanoparticles were separated from an enzymatically digested biological matrix, purified and concentrated for
analysis. The described method yields an approximate value for nanoplastic concentration in the organism (with a
limit of detection of 106 particles/organism, corresponding to 1 ng/g) and provides the chemical composition by
Raman spectroscopy. Furthermore, this method can be extended to other biological matrices and used to
quantitatively monitor the accumulation of nanoplastics in the environment and food chain.
to higher organisms (including humans) as they enter the food chain. Nanoplastics (defined here as smaller than
1 μm) are particularly challenging to detect and analyze at environmentally relevant concentrations and in
biological matrices. The tunicate Ciona Robusta is an effective bioindicator for microplastics and nanoplastic
contamination in the marine environment, due to its capacity to filter substantial volumes of water and to
accumulate particulates. In this proof-of-principle study that demonstrates a complete methodology, following
controlled exposure using spiked samples of a model nanoplastic (100 nm diameter polystyrene spheres) the
nanoparticles were separated from an enzymatically digested biological matrix, purified and concentrated for
analysis. The described method yields an approximate value for nanoplastic concentration in the organism (with a
limit of detection of 106 particles/organism, corresponding to 1 ng/g) and provides the chemical composition by
Raman spectroscopy. Furthermore, this method can be extended to other biological matrices and used to
quantitatively monitor the accumulation of nanoplastics in the environment and food chain.