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A versatile fabrication and assembly approach for microfluidic capturing of extracellular vesicles

A versatile fabrication and assembly approach for microfluidic capturing of extracellular vesicles

Category
Conference lecture and academic presentation
Abstract
Cells communicate by means of "messengers" known as extracellular vesicles, i.e. small (30‐1000 nm) cell derived vesicles released from almost all eukaryotic cells into their extracellular environment and containing a variety of proteins and RNAs [1]. It has been foreseen that the next generation of biomarkers for disease diagnostics and prognostics may strongly depend on the vesicles being efficiently isolated, quantified and analyzed by e.g. reliable, high‐throughput and possibly easy‐to‐operate microfluidic devices. The results reported here extend the previously reported work by Chen et al. [2]: (i) the fabrication approach is based on silicon as a micromolding master, which offers better dimensional control and durability than SU‐8 masters, (ii) a staggered herringbone micromixer [3] is introduced in three different channel configurations , (iii) the device assembly allows straightforward integration of a large variety of surface chemistries by introducing prefunctionalized glass slides; (iv) individual functionalization of the glass slide and the PDMS replica prior to assembly permits combination of two differently biofunctionalized surfaces within the same fluidic device; (v) isolated extracellular vesicles can be accessed directly for a variety of post‐analyses by opening the device assembly.
Language
English
Author(s)
Affiliation
  • SINTEF Digital / Microsystems and Nanotechnology
  • Norwegian University of Science and Technology
  • University of Oslo
  • University of Oslo
Presented at
Lab-on-a-chip and microfluidics conference 2015
Place
Berlin
Date
16.03.2015 - 17.03.2015
Organizer
Selectbio
Year
2015