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Magnetic-Responsive Carbon Nanotubes Composite Scaffolds for Chondrogenic Tissue Engineering

Abstract

The demand for engineered scaffolds capable of delivering multiple cues to cells continues to grow as the interplay between cell fate with microenvironmental and external cues is revealed. Emphasis has been given to develop stimuli-responsive scaffolds. These scaffolds are designed to sense an external stimulus triggering a specific response (e.g., change in the microenvironment, release therapeutics, etc.) and then initiate/modulate a desired biofunction. Here, magnetic-responsive carboxylated multi-walled carbon nanotubes (cMWCNTs) are integrated into 3D collagen/polylactic acid (PLA) scaffold via a reproducible filtration-based method. The integrity and biomechanical performance of the collagen/PLA scaffolds are preserved after cMWCNT integration. In vitro safety assessment of cMWCNT/collagen/PLA scaffolds shows neither cytotoxicity effects nor macrophage pro-inflammatory response, supporting further in vitro studies. The cMWCNT/collagen/PLA scaffolds enhance chondrocytes metabolic activity while maintaining high cell viability and extracellular matrix (i.e., type II collagen and aggrecan) production. Comprehensive in vitro study applying static and pulsed magnetic field on seeded scaffolds shows no specific cell response in dependence with the applied field. This result is independent of the presence or absence of cMWCNT into the collagen/PLA scaffolds. Taken together, these findings provide additional evidence of the benefits to exploit the CNTs outstanding properties in the design of stimuli-responsive scaffolds.
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Category

Academic article

Language

English

Author(s)

  • Muthusamy Saranya
  • Aldeliane M. da Silva
  • Hanna Karjalainen
  • Geir Klinkenberg
  • Ruth Baumberger Schmid
  • Birgitte Hjelmeland McDonagh
  • Peter Molesworth
  • Margrét Sylvía Sigfúsdóttir
  • Ane Marit Wågbø
  • Susana. G. Santos
  • Cristiana Couto
  • Ville-Pauli Karjalainen
  • Shuvashis Das Gupta
  • Topias Järvinen
  • Luisa de Roy
  • Andreas. M. Seitz
  • Mikko Finnilä
  • Simo Saarakkala
  • Anne Marie Haaparanta
  • Lauriane Janssen
  • Gabriela S. Lorite

Affiliation

  • SINTEF Industry / Biotechnology and Nanomedicine
  • Finland
  • University of Oulu
  • University of Porto
  • Germany

Year

2023

Published in

Advanced Healthcare Materials

ISSN

2192-2640

Volume

12

Issue

30

View this publication at Norwegian Research Information Repository