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Liposomes - Human phagocytes interplay in whole blood: effect of liposome design

Abstract

Nanomedicine holds immense potential for therapeutic manipulation of phagocytic immune cells. However, in vitro studies often fail to accurately translate to the complex in vivo environment. To address this gap, we employed an ex vivo human whole-blood assay to evaluate liposome interactions with immune cells. We systematically varied liposome size, PEG-surface densities and sphingomyelin and ganglioside content. We observed differential uptake patterns of the assessed liposomes by neutrophils and monocytes, emphasizing the importance of liposome design. Interestingly, our results aligned closely with published in vivo observations in mice and patients. Moreover, liposome exposure induced changes in cytokine release and cellular responses, highlighting the potential modulation of immune system. Our study highlights the utility of human whole-blood models in assessing nanoparticle-immune cell interactions and provides insights into liposome design for modulating immune responses.
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Category

Academic article

Language

English

Author(s)

  • Miriam Soledad Giambelluca
  • Elena Markova
  • Claire Louet
  • Bjørg Steinkjer
  • Rune Sundset
  • Natasa Skalko Basnet
  • Sjoerd Hak

Affiliation

  • SINTEF Industry / Biotechnology and Nanomedicine
  • UiT The Arctic University of Norway
  • University Hospital of North Norway
  • Norwegian University of Science and Technology

Year

2023

Published in

Nanomedicine: Nanotechnology, Biology and Medicine

ISSN

1549-9634

Volume

54

View this publication at Norwegian Research Information Repository