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Intravital microscopy for real-time monitoring of drug delivery and nanobiological processes

Abstract

Intravital microscopy (IVM) expands our understanding of cellular and molecular processes, with applications ranging from fundamental biology to (patho)physiology and immunology, as well as from drug delivery to drug processing and drug efficacy testing. In this review, we highlight modalities, methods and model organisms that make up today’s IVM landscape, and we present how IVM - via its high spatiotemporal resolution - enables analysis of metabolites, small molecules, nanoparticles, immune cells, and the (tumor) tissue microenvironment. We furthermore present examples of how IVM facilitates the elucidation of nanomedicine kinetics and targeting mechanisms, as well as of biological processes such as immune cell death, host-pathogen interactions, metabolic states, and disease progression. We conclude by discussing the prospects of IVM clinical translation and examining the integration of machine learning in future IVM practice.

Category

Academic literature review

Language

English

Author(s)

  • Jeffrey Momoh
  • Dimitrios Kapsokalyvas
  • Michael Vogt
  • Sjoerd Hak
  • Fabian Kiessling
  • Marc van Zandvoort
  • Twan Lammers
  • Alexandros Marios Sofias

Affiliation

  • SINTEF Industry / Biotechnology and Nanomedicine
  • Maastricht University
  • RWTH Aachen University
  • Aachen University Hospital
  • Norwegian University of Science and Technology

Year

2022

Published in

Advanced Drug Delivery Reviews

ISSN

0169-409X

Volume

189

Page(s)

1 - 21

View this publication at Norwegian Research Information Repository