To main content

Efficacy of a novel sequential enzymatic hydrolysis of lignocellulosic biomass and inhibition characteristics of monosugars

Abstract

Efficient production of sugar monomers from lignocellulose is often hampered by serious bottle-necks in biomass hydrolysis. The present study reveals that ultra-sonication assisted pretreatment following autoclaving, termed as combined pretreatment, can lead to more efficient delignification of lignocellulosic biomass and an open, deformed polysaccharide matrix, found favorable for subsequent enzymatic hydrolysis, is formed. The pattern of inhibition for the enzymatic hydrolysis reaction on combined-pretreated saw dust is identified. Two main inhibition models (competitive and noncompetitive) are proposed and a better fit of experimental values with the theoretical values for the competitive inhibition model validates the proposition that in the present experiment, glucose inhibits the enzymes competitively. Additionally, accuracy of the inhibitory kinetics based models is estimated over a series of enzyme and substrate concentrations.
Read the publication

Category

Academic article

Language

English

Author(s)

Affiliation

  • SINTEF Industry / Biotechnology and Nanomedicine
  • SINTEF Industry / Metal Production and Processing
  • Norwegian University of Science and Technology
  • India
  • Jadavpur University

Year

2019

Published in

International Journal of Biological Macromolecules

ISSN

0141-8130

Volume

129

Page(s)

634 - 644

View this publication at Norwegian Research Information Repository