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Hands-free sample preparation platform for nucleic acid analysis

Abstract

A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.
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Category

Academic article

Language

English

Author(s)

  • Tobias Baier
  • Thomas Hansen-Hagge
  • Rainer Gransee
  • A. Crombe
  • S. Schmahl
  • C. Paulus
  • K.S. Drese
  • H. Keegan
  • C. Martin
  • J. J. O'Leary
  • Liv Furuberg
  • L. Solli
  • P. Grønn
  • I. Falang
  • Aina Karlgård
  • Anja Gulliksen
  • F Karlsen

Affiliation

  • Unknown
  • SINTEF Digital / Smart Sensors and Microsystems

Year

2009

Published in

Lab on a Chip

ISSN

1473-0197

Publisher

Royal Society of Chemistry (RSC)

Page(s)

3399 - 3405

View this publication at Cristin