Abstract
The activation of phospholipase A2 (PLA2) by means of pro-inflammatory
cytokines results in the subsequent release of arachidonic acid (AA)
and generates eicosanoids, which further propagate inflammation. Through
cyclooxygenases (COX1/2) responsible for the enzymatic conversion of AA to
eicosanoids, non-steroidal anti-inflammatory drugs are effective in relieving
the pain and discomfort of inflammation. Using the AA release assay as a
guide for biological and anti-inflammatory activity, the novel compound 1-O-
-(3-O-linolenoyl-6-deoxy-6-sulfo-α-D-glucopyranosyl)glycerol (1) together
with five known compounds, i.e., isovitexin, byzantionoside B, tricin 4′-O-
-(erythro-β-guaiacylglyceryl) ether 7-O-β-glucopyranoside, 1-O-feruloylglycerol
and tricin 7-glucoside, isolated from the methanol extract of the aerial
parts of Sclerochloa dura using LC techniques (Sephadex LH-20 column chromatography,
preparative HPLC and semi-preparative HPLC), were evaluated.
All the isolated compounds were identified using spectroscopic methods, i.e.,
NMR spectroscopy and MS spectrometry. The novel compound 1 was found to
be an effective inhibitor of AA release with an IC50 value of 0.09±0.03 mg mL-1.
cytokines results in the subsequent release of arachidonic acid (AA)
and generates eicosanoids, which further propagate inflammation. Through
cyclooxygenases (COX1/2) responsible for the enzymatic conversion of AA to
eicosanoids, non-steroidal anti-inflammatory drugs are effective in relieving
the pain and discomfort of inflammation. Using the AA release assay as a
guide for biological and anti-inflammatory activity, the novel compound 1-O-
-(3-O-linolenoyl-6-deoxy-6-sulfo-α-D-glucopyranosyl)glycerol (1) together
with five known compounds, i.e., isovitexin, byzantionoside B, tricin 4′-O-
-(erythro-β-guaiacylglyceryl) ether 7-O-β-glucopyranoside, 1-O-feruloylglycerol
and tricin 7-glucoside, isolated from the methanol extract of the aerial
parts of Sclerochloa dura using LC techniques (Sephadex LH-20 column chromatography,
preparative HPLC and semi-preparative HPLC), were evaluated.
All the isolated compounds were identified using spectroscopic methods, i.e.,
NMR spectroscopy and MS spectrometry. The novel compound 1 was found to
be an effective inhibitor of AA release with an IC50 value of 0.09±0.03 mg mL-1.