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Metabolic fingerprinting of arctic copepods Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus

Abstract

Recent development in high-throughput unbiased analytical tools (transcriptomics, proteomics and metabolomics) has opened the possibility to assess a multitude of molecular endpoints in organisms. In the present work, we used a combination of metabolomics tools, proton nuclear magnetic resonance spectroscopy (1H-NMR) and liquid chromatography coupled with mass spectrometry (LC–MS),) and multivariate statistical analysis to identify species-specific metabolome fingerprints and species-specific metabolites in the three Arctic copepods Calanus finmarchicus, C. glacialis and C. hyperboreus. Principal component analysis separated the three species with high specificity and sensitivity, and some species-specific metabolites were putatively annotated. These tools can be used for future studies within basal biology, systems biology, bioprospecting and ecotoxicology. As a supplementary analytical tool to genetic analyses, species-specific metabolites have a potential to be used to separate closely related Arctic Calanus species from net hauls.

Category

Academic article

Language

English

Affiliation

  • SINTEF Ocean / Miljø og nye ressurser
  • SINTEF Industry / Biotechnology and Nanomedicine
  • Diverse norske bedrifter og organisasjoner

Year

2013

Published in

Polar Biology

ISSN

0722-4060

Publisher

Springer

Volume

36

Issue

11

Page(s)

1577 - 1586

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