We have so far established three Calanus finmarchicus EST libraries, consisting of nearly 3000 ESTs. These have been BLASTed, and we have discovered unique sequences. After BLAST alignments towards crustacean genomic sequences in GenBank we have found highly similar matches, and of these we have now clones of ESTs from some very interesting genes in a toxicological sense. Some of these are listed below:
We are continuously uploading ESTs to NCBI GenBank, and the complete list is found under "Submission to GenBank".
We are continuously working to develop real-time PCR-methods to to quantify the expression of specific mRNAs during exposure to different types of stressors (oil compounds, heavy metals, amines, elevated temperature and more).
Published March 31, 2007
Calanus finmarchicus - a new and relevant test species for assessing effects of toxicants and climate changes in the marine environment.