Biological effects assessment
Testing of "old and new" chemicals should include assessment of biological effects on low exposure concentrations, similar to those relevant in the environment. . This test is used to indicate the potential for long-term effects of chemicals (or mixtures, like water soluble fractions of oil) using a short-term (12, 24 and 48 hours) exposure to low levels (0.5, 5 and 50% of LC50-concentrations) of chemicals.

We use static, semi-static and flow-through exposure systems, usually with serially diluted stock solutions of a chemical/mixture. The concentrations used are always determined on the basis of an initial acute toxicity test.

After exposure, copepods are sampled and analyzed for expression of a battery of genes (by qPCR or (soon) microarray) relevant for indicating chronic effects. We may also supply with other endpoints indicating potential for endocrine disruption, cytotoxicity and DNA damage.

Published February 27, 2008

Calanus finmarchicus - a new and relevant test species for assessing effects of toxicants and climate changes in the marine environment.