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Molecular biology laboratory

Molecular biology laboratory

Published 09 February 2016

We have state of the art equipment for molecular biology, including an Illumina MiSeq sequencer, droplet digital PCR (ddPCR), Agilent Bioanalyzer, CHEF Mapper XA pulse field gel electrophoresis system and a Bluepippin cassette-based gel electrophoresis system for DNA and protein separation, in addition to ÄKTA FPLC equipment for protein purification. Our co-workers have several years of experience and master most topics and protocols related to genetic modification of bacteria.

Techniques and research areas

  • Recombinant strain development for production of proteins, polysaccharides, and metabolites with an industrial potential
  • Enzyme evolution through mutagenesis and screening
  • Standard molecular biology techniques (Isolation and quantification of DNA, RNA and/or proteins, restriction digestion, sub-cloning, ligation, PCR, cDNA synthesis, separation by gel electrophoresis or FPLC, SDS-PAGE, Southern-blot, Western-blot, Northern-blot, assembly cloning (SLIC, Gibson assembly etc.), transformation of Escherichia coli and other bacteria, (conjugation from E. coli)
  • Library cloning and cloning of large DNA fragments (BAC, fosmids) and screening (at the HTS laboratory)
  • Taxonomic population studies by amplicon sequencing
  • Largescale Illumina Sequencing (genome sequencing, metagenome sequencing), assembly, and annotation
  • Transcriptome analysis (RNASeq, microarray)
  • Enzyme assays
Research Manager

Contact

Håvard Sletta, Cellphone: 915 44 429, Email: Havard.Sletta@sintef.no

Molecuelar biology laboratory,
Realfagbygger, room D2-199, Høgskoleringen 5, 7034 Trondheim